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Real-Time PCR (BIOS Advanced Methods) (Englisch) Taschenbuch – 27. April 2006

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Real-time PCR is based on the conventional principles of PCR and, with the simple shift of emphasis from the end-product to the whole course of the PCR process, has established itself as the most sensitive and specific quantitative PCR method. Real-time PCR is, like any other modern method in molecular genetics, expanding, with potential applications even in proteomics. With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. "Real-Time PCR" provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It doesn't cover every aspect of real-time PCR, but addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the "Advanced Methods Series", most chapters provide an experimental protocol as an example of a specific assay.

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Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK

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There are several ways to interrogate a cell for changes induced by artificial or natural agents during a biological process. Lesen Sie die erste Seite
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6 von 6 Kunden fanden die folgende Rezension hilfreich
Good Resource 29. Oktober 2006
Von John - Veröffentlicht auf
Format: Taschenbuch
This is a good guide for getting started; I highly recommend it to graduate students or others diving into real time for the first time. It's also nice to have around as a reference when you start something new or need a refresher on data analysis and interpretation. I'm glad I bought it.
4 von 4 Kunden fanden die folgende Rezension hilfreich
Good introduction 18. Mai 2009
Von Dreverhaven - Veröffentlicht auf
Format: Taschenbuch
This is an excellent book for starters, like myself, in the technique of Real-time PCR, and it will not disappoint more advanced users who wish to refresh their knowledge or learn alternative meethods or correction strategies that apply to their protocols.
In attempting to be comprehensive, it may sometimes sound superficial when it mentions an aspect of the subject without delving deeper into it. This is especially true of the chapters on normalization and statistical analysis, where the reader is often referred to Stephen Bustin's A-Z of Quantitative PCR. Incidentally, Bustin himself is a co-author of this book.
All in all, it helps a lot in giving self-confidence to scientists who wish to know whether their analysis of quantitative data is correct. I carry my copy with me to the lab and consult it whenever I want to know whether I can rule out background noise or differences in efficiency in fold changes for my experiments, for example. The explanations on the different chemistries and the study of amplification curves is very attentive to detail and one never gets lost in equations. The part on melt curve analysis for SYBR protocols is unfortunately shallow, but this is a minor flaw in an overall good introductory book.
Another point in favour of this purchase is the care the authors take to provide examples of protocols, step by step, including volumes and concentrations, in the end of each chapter. They also supply examples of useful sites in the Internet that supply analysis tools, and do not refrain from citing brands and models of thermal cyclers, discussing briefly their signal detection methods.
One should not be fooled by its publication date, though. They say 2009, but it is only an unmodified reprint of the 2006 edition. Also, only the first eight chapters are dedicated to the technique as a whole: the remaining are protocols and discussions on specific applications that will interest those who perform similar analyses.
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