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Any basic research laboratory using molecular biology techniques will benefit from having a copy on hand of the newly published Third Edition of Molecular Cloning: A Laboratory Manual, by Joe Sambrook and David Russell... Originally published almost 20 years ago, the first two editions of this book have been staples of molecular biology with a proven reputation for accuracy and thoroughness. The Scientist Molecular Cloning: A Laboratory Manual has always been the laboratory mainstay for protocols and techniques. It has a pure-bred ancestry, and the new addition does not disappoint. Sambrook and Russell have built upon the basic principles that made the first two editions essential reference resources. Protocols that cover everything possible in a molecular biology lab are explained in detail. ...The new edition includes information panels at the end of each chapter that describe the principles behind the protocols... The addition of this information extends Molecular Cloning from an essential laboratory resource into a new realm, one merging the previous prototype with a modern molecular monograph... It is difficult to find fault with the first two editions of Molecular Cloning. For decades, their superior presentation of laboratory protocols made them the gold standard in the field of molecular biology. However, in many laboratories the old editions are becoming worn out-a testament to their continual use. For most, it is time to retire these workhorses and allow them some peace. They and you will be safe in the knowledge that the next generation of Molecular Cloning not only carries on the proud heritage of the first two editions but also admirably expands on that tradition to provide a truly essential laboratory manual. Trends in Microbiology In every kitchen there is at least one indispensable cookbook. Sambrook and Russell's Molecular Cloning: A Laboratory Manual fills the same niche in the laboratory. Like its kitchen counterparts (e.g. Rombeck's Joy of Cooking) Sambrook's Molecular Cloning (MC) has information to help both the inexperienced and the advanced user. The analogy is apt, as MC is set up, and reads, very much like a molecular cookbook. Like a good cookbook, it has good logistics. Moreover, like 'Joy', the authors provide a wealth of theoretical (and historical) background for each experimental paradigm. MC is well organized, with a thorough index and detailed table of contents... MC provides the user with detailed information about the characteristics of reagents, supplies and kits from various manufacturers-all of which is important in our age of 'kit science'. The authors point out the pros and cons involved in using such reagents and kits, and recommend them at times (e.g. for cDNA synthesis and library construction), but stress caution in others (e.g. switching to non-radioactive detection methods). We applaud the inclusion of protocols even when kits are suggested, so users can be aware of the traditional methods that accomplish the same tasks... with this new edition and its companion website, MC has once again established its primacy as the molecular laboratory manual and is likely to be found on lab benches, and book-marked on web browsers, around the world. Trends in Neurosciences
Kurzbeschreibung
The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today's biomolecular techniques. The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
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THIS PRINT EDITION OF MOLECULAR CLONING is associated with a Web Site (www.MolecularCloning.com) that is evolving into an on-line laboratory manual. Lesen Sie die erste Seite
Buchdeckel | Copyright | Inhaltsverzeichnis | Auszug | Stichwortverzeichnis | Rückseite
THIS PRINT EDITION OF MOLECULAR CLONING is associated with a Web Site (www.MolecularCloning.com) that is evolving into an on-line laboratory manual. Lesen Sie die erste Seite
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Kundenrezensionen
Die hilfreichsten Kundenrezensionen
5 von 5 Kunden fanden die folgende Rezension hilfreich
Format:Gebundene Ausgabe
Ein Muß für alle Labore, die mit recombinant DNA technology arbeiten. Einfach einzigartig und unerreicht - die Genauigkeit und die nützlichen Querverweise.
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18 Rezensionen
24 von 24 Kunden fanden die folgende Rezension hilfreich
Excellent reference for all
9. März 2002
Format:Taschenbuch
In this 3 volume set of books the authors summarize the most important laboratory protocols for DNA analysis and cloning. As someone involved in computational biology and mathematical gene sequence analysis, I was needing such a summary to get an idea of just how genetic engineering is actually practiced in the laboratory. The book is definitely written for those readers that are very experienced in these "wet" techniques, but it still could be perused profitably by anyone who is curious about genetic engineering. There is also an excellent website that owners of the books can go to and search for protocols and obtain updates and additions to the protocols.
At the beginning of each chapter, the authors give an introduction to the protocols and this is of an enormous help to those readers with only rudimentary acquaintance with the laboratory procedures. Typically, this introduction contains an historical summary of the procedures as they were developed or discovered. One can only marvel at the ingenuity of the discoverers of these techniques. These introductions are fairly straightforward to read, even for those that are not experts in biochemistry.
At the end of each chapter, the authors include an "information panel" that gives a more in-depth view of the biochemistry or genetics behind the procedures. These are summaries and are highly specialized, and are again meant for experienced readers. A very lengthy list of references is also included at the end of each chapter.
Becuase of the size of this collection, space here does not permit a detailed review, so I will list some of the areas that I thought were particularly interesting or well-written (these coming from the introduction or the information panels only): 1. The DNA synthesis at the colE1 replicon and the interaction between RNAI and RNAII. 2. The discussion of electroporation and the physics behind this technique to introduce DNA into eukaryotic cells. 3. The discussion on the discovery of bacteriophage lambda. 4. The discussion (with diagram), of the assembly pathway of bacteriophage lambda. 5. The summary of the early analysis of DNA using electrophoresis and the different pulsed-field configurations used. 6. The anecdote on the discovery of the polymerase chain reaction. 7. The short discussion on computer-assisted design of oligonucleotide primers. 8. The discussion of oligonucleotide synthesis. 9. The flowchart detailing the preparing and screening of a cDNA library. 10. The history of the development of the methods to synthesize and clone cDNAs. 11. The detailed discussion of the molecular cloning of double-stranded cDNA. 12. The discussion on the methods to validate clones of cDNA. 13. The discussion on magnetic beads for affinity purification. 14. The discussion on the history of DNA sequencing and the different techniques to accomplish it, particularly the information panel on automated DNA sequencing. 15. The discussion of the different types of mutagenesis and the different methods for accomplishing it. 18. The fascinating discussion of how to introduce cloned genes into mammalian cells. 19. The discussion on the steps involved in DNA footprinting. 20 The discussion on green flourescent protein and its use as a fusion tag. 21. The discussion on the use of surface plasmon resonance spectroscopy.
15 von 15 Kunden fanden die folgende Rezension hilfreich
a formerly essential classic
23. November 2005
Format:Taschenbuch
For many years the previous edition of this set was an essential reference in molecular biology labs. At present however, there are too many good protocol books out there to really make this argument. The book is pretty strong in explaining theory, and answering the question of why certain procedures are either necessary, useful, or worthless, however it is not as practical as many other books, such as Short Protocols. Still a good reference overall, but no longer stands alone, and I recommend checking out as much of the competition as possible before deciding whether to make the investment in it.
10 von 12 Kunden fanden die folgende Rezension hilfreich
The bible of molecular cloning-updated
14. August 2001
Format:Taschenbuch
Molecular cloning has been a lab staple for years. Now reprinted so you can update the old lab copy worn out by years of student use! Its a must have for any lab serious about molecular biology. Its also useful for student training. Many times there are simple explanations for the lab techniques we have adopted as dogma, but are unsure why. Molecular cloning has the answers and is a great resource. I highly recommend this book for its depth and breadth of protocols and guidance in the complicated realm of cloning!
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